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         [KO Validated] LC3B Rabbit mAb
        

        
產(chǎn)品貨號(hào)：A19665
        
產(chǎn)品品牌：ABclonal
        
產(chǎn)品分類：抗體
        
產(chǎn)品規(guī)格：50ul 100ul 200ul
        
產(chǎn)品價(jià)格：1250元 1950元 3200元

        


        

        


                    
        產(chǎn)品介紹
        
                   
                    
        
                        
        背景
        The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
        免疫原信息
        應(yīng)用
        反應(yīng)物種Human, Mouse, Rat
        驗(yàn)證應(yīng)用WB IHC IF
        用戶驗(yàn)證的應(yīng)用
        推薦稀釋比&實(shí)驗(yàn)步驟
        • WB   1:500 - 1:2000  [ 實(shí)驗(yàn)步驟 ]
        • IHC   1:50 - 1:200  [ 實(shí)驗(yàn)步驟 ]
        • IF   1:50 - 1:200  [ 實(shí)驗(yàn)步驟 ]
        理論分子量14kDa,16kDa
        實(shí)際分子量14kDa,16kDa
        存儲(chǔ)緩沖液Store at -20℃. Avoid freeze / thaw cycles.
        Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
        實(shí)驗(yàn)方法Western blotting    
        陽性樣本HeLa,293T,C6,NIH/3T3
        細(xì)胞定位Cytoplasm,Cytoplasmic vesicle,Endomembrane system,Lipid-anchor,autophagosome,autophagosome membrane,cytoskeleton
        純化方式Affinity purification
        [KO Validated] LC3B Rabbit mAb 檢測(cè)圖片
        ABclonal:Western blot - [KO Validated] LC3B Rabbit mAb (A19665) }
        Western blot - [KO Validated] LC3B Rabbit mAb (A19665)
        Western blot analysis of extracts of various cell lines, using LC3B antibody (A19665) at 1:1000 dilution.293T cells, LC3B knockout (KO) 293T cells, C6 cells and NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
        Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
        Lysates/proteins: 25ug per lane.
        Blocking buffer: 3% nonfat dry milk in TBST.
        Detection: ECL Basic Kit (RM00020).
        Exposure time: 5s.
        ABclonal:Western blot - [KO Validated] LC3B Rabbit mAb (A19665) }
        Western blot - [KO Validated] LC3B Rabbit mAb (A19665)
        Western blot analysis of extracts of Hela cells, using LC3B pAb (A19665) at 1:1000 dilution.Hela cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
        Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
        Lysates/proteins: 25ug per lane.
        Blocking buffer: 3% BSA.
        Detection: ECL Basic Kit (RM00020).
        Exposure time: 5s.
        ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665) }
        Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)
        Immunohistochemistry of paraffin-embedded Rat brain using LC3B antibody (A19665) at dilution of 1:100 (40x lens).
        ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665) }
        Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)
        Immunohistochemistry of paraffin-embedded Human brain using LC3B antibody (A19665) at dilution of 1:100 (40x lens).
        ABclonal:Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665) }
        Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)
        Immunohistochemistry of paraffin-embedded Mouse spinal cord using LC3B antibody (A19665) at dilution of 1:100 (40x lens).
        ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665) }
        Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)
        Immunofluorescence analysis of C6 cells, untreated(right) and Chloroquine treated(50 μM, 37℃ for 20 hours;left) using LC3B antibody (A19665) at dilution of 1:100. Blue: DAPI for nuclear staining.
        ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665) }
        Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)
        Immunofluorescence analysis of HeLa cells, untreated(right) and Chloroquine treated(50 μM, 37℃ for 20 hours;left) using LC3B antibody (A19665) at dilution of 1:100. Blue: DAPI for nuclear staining.
        ABclonal:Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665) }
        Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)
        Immunofluorescence analysis of NIH/3T3 cells, untreated(right) and Chloroquine treated(50 μM, 37℃ for 20 hours;left) using LC3B antibody (A19665) at dilution of 1:100. Blue: DAPI for nuclear staining.